You’ve done it. You’re kid’s finally not scared of the tub, gets the time of his life during bath, splashing water on himself and on you and sees bath time as a great opportunity to play, wonder and explore. However, your job as parent regarding bath time does not end in making it a fun time for your kid. More importantly, it is also your responsibility to ensure his safety during baths. Safety Tip 1: Be prepared. Everything should be ready. His towel, soap, shampoo, ba...

The north is cold, the south is warm, right? Wrong. It's not that simple...even in the northern hemisphere.

The hypothesis was supported by the data. After increased exposure to plasmid pAMP/pBlu, the transformation efficiency increased. There were .4*10^2 colonies per mg of plasmid. One hundred and eighty colonies were found in the LB- agar plate with no ampicillin or plasmid. Two colonies were found on the LB/AMP+ plate with ampicillin and plasmid.
The results of this experiment may be inconclusive due to the following errors: The competent cells could have been damaged in the process of making them competent. The heat bath was to be set exactly at 42 degrees Celsius. Any higher could have damaged the cells making them incompetent and unable to pick up foreign DNA from the plasmid. The cells may have been left in the ice bath too long damaging the cell wall causing them also to not be able to pick up foreign DNA from the plasmid. If the cells were not able to pick up DNA, then they could not be transformed, sharply decreasing the transformation efficiency. A final error possibly could have been that there were not enough e-coli to transform. Since the group decided to use e-coli from their own throat, the number of cells transferred may have been minimal. This would also cause a decrease in the transformation efficiency.